Journal: Journal of the American Society of Nephrology

Article Title: Complement Activation Regulates the Capacity of Proximal Tubular Epithelial Cell to Stimulate Alloreactive T Cell Response

doi: 10.1097/01.asn.0000135974.06478.7b

Figure Lengend Snippet: Figure 6. Detection of IL-2 and IFN- by ELISA. IFN-–stimulated PTEC were treated with NS or HIS for 30 min and subsequently co-cultured with T cells. (A and B) Supernatants were collected from co-culture of 2 105 PTEC with 2 105 T cells at various time points and assayed for IL-2 and IFN- by sandwich ELISA. P values are for comparisons between NS and HIS treatment. *P 0.05; **P 0.005; ***P 0.0003.

Article Snippet: Sandwich ELISA was performed using ELISA Kit for mouse IFN- or IL-2 (R&D Systems, Oxford, UK) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Co-Culture Assay, Sandwich ELISA

Journal: Journal of the American Society of Nephrology

Article Title: Complement Activation Regulates the Capacity of Proximal Tubular Epithelial Cell to Stimulate Alloreactive T Cell Response

doi: 10.1097/01.asn.0000135974.06478.7b

Figure Lengend Snippet: Figure 7. Relative involvement of CD4 and CD8 T cells in complement-enhanced alloreactive T cell response. IFN-–stimulated and NS- or HIS-treated PTEC were co-cultured with purified allo- reactive CD4 or CD8 T cells. Supernatants were collected from co-culture of PTEC with T cells after 72 h of co-culturing and assayed for IFN- by sandwich ELISA. P values are for comparisons between NS and HIS treatment. **P 0.005.

Article Snippet: Sandwich ELISA was performed using ELISA Kit for mouse IFN- or IL-2 (R&D Systems, Oxford, UK) according to the manufacturer’s instructions.

Techniques: Cell Culture, Purification, Co-Culture Assay, Sandwich ELISA

Journal: Journal of the American Society of Nephrology

Article Title: Complement Activation Regulates the Capacity of Proximal Tubular Epithelial Cell to Stimulate Alloreactive T Cell Response

doi: 10.1097/01.asn.0000135974.06478.7b

Figure Lengend Snippet: Figure 8. Complement-mediated PTEC stimulation of alloreactive T cell response can be inhibited with CTLA4-Ig. IFN-–stimulated and NS- or HIS-treated PTEC were co-cultured with purified alloreactive CD4 T cells in medium alone or in the presence of CTLA4-Ig (5 g/ml) or control Ig (human IgG1, 5 g/ml). Supernatants were collected from co-culture of PTEC with T cells after 72 h of co- culturing and assayed for IFN- by sandwich ELISA. P values are for comparisons between CTLA4-Ig treatment and medium alone or control Ig treatment. **P 0.005.

Article Snippet: Sandwich ELISA was performed using ELISA Kit for mouse IFN- or IL-2 (R&D Systems, Oxford, UK) according to the manufacturer’s instructions.

Techniques: Cell Culture, Purification, Control, Co-Culture Assay, Sandwich ELISA

Journal: Oncoimmunology

Article Title: Systemic administration of β-glucan of 200 kDa modulates melanoma microenvironment and suppresses metastatic cancer.

doi: 10.1080/2162402X.2017.1387347

Figure Lengend Snippet: Figure 3. BG34-200 treatment augments systemic activation of T cells recognizing melanoma antigen. (A) Splenocytes or TDLN cells were stimulated with gp10025-33 peptide. IFN-g and IL-2 levels in the culture media were assessed using ELISA. B. Frequencies of CD3, CD4, CD8 T cells and their expression of CD62L and CD44 in spleen and TDLN were determined by FACS analysis. C. TDLN cells were subjected to intracellular cytokine staining for determining the frequency of gp10025-33 -specific T cells. (A) and (B) were graphed as means ± SD. Each data point represents one of three replicates of samples from individual TDLN or spleen. * p < 0.05, ** p < 0.01. Significance was determined using Student’s t test.

Article Snippet: Mouse IFN- and IL-2 ELISA kits were purchased from R&D Systems (Minneapolis, MN).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Expressing, Staining